Carnosine facilitates nitric oxide production in endothelial F-2 cells
Laboratory studyOther animals
- Design
- Laboratory study
- Dose used in the study
- Concentrations above 5 mM in culture
- What was measured
- Nitric oxide production in F-2 endothelial cells, with NOS inhibitors and calcium manipulation.
What the authors reported
Carnosine increased nitric oxide production in a dose-dependent manner at concentrations above 5 mM. The effect was blocked by L-NAME but not D-NAME, and beta-alanine, histidine and anserine did not increase nitric oxide. Carnosine did not induce eNOS phosphorylation at Ser1177. The increase occurred without extracellular calcium but was abolished by depleting intracellular calcium with BAPTA, and intracellular calcium rose after about 4 minutes. The authors suggest eNOS is activated by calcium released from intracellular stores.
Limits of this study
Cell line; millimolar concentrations. Full text not read.
Source
PubMed 19881293 · doi:10.1248/bpb.32.1836
Entry checked against PubMed abstract (E-utilities) on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.