HNE Michael adducts to histidine and histidine-containing peptides as biomarkers of lipid-derived carbonyl stress in urines: LC-MS/MS profiling in Zucker obese rats
Laboratory studyOther animals
- Design
- Laboratory study
- What was measured
- LC-MS/MS detection and quantification of HNE adducts with histidine and carnosine in urine of Zucker obese rats.
What the authors reported
Three adducts excreted in free form were identified, including carnosine-HNE, which had been proposed in earlier in vitro work as a biomarker of carbonyl stress; free histidine-HNE was found only in protein hydrolysates. A validated MRM method quantified these adducts in urine with limits of quantification from 0.01 nmol/mL, and the authors propose the method for testing carbonyl-quenching agents in vivo.
Limits of this study
Method development in an obese rat model; no intervention tested. Abstract only.
Source
PubMed 17979257 · doi:10.1021/ac7016184
Entry checked against PubMed abstract (E-utilities) on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.